We are working ona MCF7 xenograft model and will try to supplement estrogen by either E2 pellet implantation or E2 in drinking water. However, most of the papers did not mention the timing that how many days do they provide E2-containing water for the mice before the implantation of MCF7 tumor cells. Does anyone have experience on this condition? We will prepare the E2 containing drinking water according to the paper listed below.
Biology Of Reproductions 35, 1088-1095 (1986)
Hi I normally use E2 pellet implants that last for 60 days. I have never used E2 in water. I have used other drugs in drinking water and I normally add to drinking water at least one week, prior to injecting cells.
E2 Pellet is better compared to supplimentation in water. You can start supplimentation (4micromolar) one week prior to cell inoculation.It worked in our lab.
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