How do you reduce the gelling time of 4 arm peg maleimide crosslinked with peg dithiol?

I'm trying to slow down the gelling of 4 arm peg maleimide crosslinked with peg dithiol. I'm using (at the moment) 20 kDa 4 arm peg maleimide + 1 kDa Peg Dithiol. the buffer solution i'm using is...

02 March 2015 5,060 3 View

What is the effect of the Molecular weight of a crosslinker and crosslinking density on the mesh size of a hydrogel?

I'm trying to develop a hydrogel which can release proteins at a controlled rate. I'm currently using 4 arm PEG maleimide (10 & 20 kDa) and 2 kDa PEG dithiol has the crosslinker via micheal...

02 March 2015 9,923 2 View

Is 20kDa PEGDA ((10% wt) 100mg in 800ul pbs) + 2kDa PEG-dithiol (10mg in 200ul PBS) stoichiometrically balanced?

is 20kDa PEGDA ((10% wt) 100mg in 800ul pbs) + 2kDa PEG-dithiol (10mg in 200ul PBS) stoichiometricly balanced? is the molar ratio (1:1) for DA:SH I attend to make the gels by michael type...

01 February 2015 1,446 5 View

What is the stokes radii of VEG-C growth factor?

I'm looking into controlled delivery of VEGF-C via PEG DA hydrogel. I know how to determine the mesh size of the gel but I want to determine the Stokes Radii of VEGF-C. thank you.

01 February 2015 9,380 1 View

How do I trypsin-digest gelatin co-polymerised with polyacrylamide gels?

31 December 2014 9,951 9 View

How can I work out the specific activity of MMP-9 enzymes theoretically?

07 August 2014 3,165 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View