Does anyone have experience of using 4 arm 2kDa PEG MAL sold by creativepeg?

03 April 2015 8,435 0 View

How do you reduce the gelling time of 4 arm peg maleimide crosslinked with peg dithiol?

I'm trying to slow down the gelling of 4 arm peg maleimide crosslinked with peg dithiol. I'm using (at the moment) 20 kDa 4 arm peg maleimide + 1 kDa Peg Dithiol. the buffer solution i'm using is...

02 March 2015 5,060 3 View

What is the effect of the Molecular weight of a crosslinker and crosslinking density on the mesh size of a hydrogel?

I'm trying to develop a hydrogel which can release proteins at a controlled rate. I'm currently using 4 arm PEG maleimide (10 & 20 kDa) and 2 kDa PEG dithiol has the crosslinker via micheal...

02 March 2015 9,923 2 View

Is 20kDa PEGDA ((10% wt) 100mg in 800ul pbs) + 2kDa PEG-dithiol (10mg in 200ul PBS) stoichiometrically balanced?

is 20kDa PEGDA ((10% wt) 100mg in 800ul pbs) + 2kDa PEG-dithiol (10mg in 200ul PBS) stoichiometricly balanced? is the molar ratio (1:1) for DA:SH I attend to make the gels by michael type...

01 February 2015 1,446 5 View

How do I trypsin-digest gelatin co-polymerised with polyacrylamide gels?

31 December 2014 9,951 9 View

How can I work out the specific activity of MMP-9 enzymes theoretically?

07 August 2014 3,165 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View