I think it's not required

Dear Manish! What do you mean?

You would need to specify what you really want to do and what you work with. I know that cells have different demandings on which proteins they need for adhesion. I worked with the Variety Pack II from BD Sciences. It contains five different coatings: Collagen I (rat tail), Fibronectin (human blood plasma), Laminin (mouse tumour), Collagen IV (mouse tumour) and Poly-DLysine (synthetic). I had 5 different cell lines and they all act really different on the surfaces. But there is a commercial product called "Matrixgel" available which is a mixture of gel and proteins which gives you a good start. Normally its used for 96-well-plate or other plastic materials. I dont know how it works on electrodes or gold surfaces. So I would recommend you to try it first in a culture plate for your cells.

And then you have to consider like Yongbai Yin mentioned, that the covering of the electrode with any kind of product will change the properties of your electrode, so it depends what measurement you use. Also the speed of the growth of your cells depends on your cells and on how much they like the surface :)

Good luck!

It is very dependent on the cell type and what information you are after. E.g. are you doing cell adhesion studies or looking at antibody binding to receptors in the cell membrane?

Dear Teodor

I used breast cancer cell MDA 231. Our purpose is reduced the time cell attach on gold electrode surface in several hours instead of days like in some literature.

Thank you so much

Anh

Hi Tien,

It depends whether you want to fix the cells, to immobilize the cells, or capture the cells with their specific antibodies.

So, kindly elaborate more information to give you the appropriate advice/answer.

Good luck,

Rabeay