17 Questions 16 Answers 0 Followers
Questions related from Tien Anh Nguyen
Dear all Could you help me by answering the question or sharing me literature to explain the impedance phase change in using ECIS cell-based biosensor for monitoring cell attachment and...
09 August 2015 5,046 2 View
Do you have any experience immobilizing DTSSP on a gold electrode to improve adsorption of protein (Laminin or Fibronectin)? How does it work or is there any literature related to this topic?
21 August 2014 5,319 3 View
I would like to test the response of cancer cells (MDA and MCF7) on Cisplatin. I bought a bottle (15g) in powder. Could you tell me how should I dilute it? Which concentration should I use? How...
16 June 2014 9,485 6 View
I would like to test Doxycycline or Cisplatin anticancer drugs with a single MDA-MB-231 breast cancer cell in our chip. Which concentration should I use and how long after treating should I...
13 May 2014 9,296 3 View
I am using MDA-MB-231 and MCF7 for doing experiment. We have CO2 independent medium. Could you tell me how should I add other components (FBS, Glutamin/max, Penicillin, Insulin..). If I need 1ml...
21 March 2014 880 2 View
I have SU8 structure on top of Au electrode? If I put the chip in the oven, at which maximum temperature can I set up which SU8 structure will not damage?
18 October 2013 8,390 2 View
I would like to record the SEM image of a cell on top of a Gold electrode? How should I prepare the sample? If you have experience, please let me know. Thank you so much
09 August 2013 4,144 1 View
I would like to modify our Gold electrode by Fibronectin or Poly-L_Lysin to reduce the time for cell attach on surface of electrode. If you have experiences, please help me.
23 May 2013 3,419 2 View
I would like to use Matrigel for cell culture but I don't know which concentration of Matrigel to use. Can anyone give some advice?
08 May 2013 6,482 2 View
I would like to use protein for reducing the time for breast cancer cell attach on the gold electrode.
30 April 2013 5,052 7 View
After finish fabricating, I coated a thin layer of AZ1518 to protect the gold electrode for dicing. Before bonding, chips were already cleaned with Axetol in ultrasonic for 3 min, isopropanol for...
15 April 2013 6,085 17 View
Could you tell me about the process to clean gold electrode before doing experiment with cells? Our system consists of thin film gold (100nm) electrode inside microchannel by SU-8. Thank you so much
10 April 2013 5,208 3 View
I have three electrodes: WE, CE and RE. How can I connect with SI 1260 to measure V1/V2?
15 March 2013 7,543 1 View
Is it necessary to do SAM before Fibronectin? With this modification, how long does it take for cells to spread on top of an electrode?
06 March 2013 4,129 6 View
My system consists of two gold electrodes inside a microchannel (300 micrometer width and 50 micrometer height) covered by a medium for cells. The surface of electrodes is modified with SAM and...
05 February 2013 8,060 0 View
I measured impedance between 2 planar gold electrodes inside a microchannel filled with medium for cell culture. Which electrical elements should I put in the circuit and how are they connected...
05 February 2013 7,956 5 View
Our system consist of two planar electrodes and a medium for cell culture.
29 January 2013 565 0 View
