I have lyophilized CEE that then must be resuspended in ddH2O. CEE is one of the components of a cell culture medium. I have a problem in filtering it alone and also as a component of that medium. I have used 0,22 and 0,45 um PVDF filters and in both cases I have the block of the filters, change filters everytime they block and at last lose a lot of material. I have also tried cell strainers of 40 and 70 um. The solution goes through but the strainers do not hold the particles.
How could I solve my problem? Do you have any suggestions for me?
Ciao Carla,
we normaly do not lyophilize CEE, we snap freeze into liquid nitrogen, nevertheless filtering is quiet impossible as you seen, we rather centrifuge in hereus at 3000rpm. otherwise in eppendorf at 13.000 rpm in order to pellet all the debris collecting supernatant and using this as supplement.
I hope this will help you, good luck.
Ciao Cesare ;-)
yes, it is rather impossible to filter it... I will centrifuge it and fingers crossed!
Thank u very much 4 your suggestion!
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