22 September 2016 5 9K Report

I would like to check the expression of two housekeeping genes, which, based on RT-PCR results, are expressed throughout development (most likely in a ubiquitous manner). Yet I just can't make the in situs to work. I tried to clone different bits of the cDNAs for probe synthesis, and used different temperatures (60C/65C/70C) for hybridization, but there is no staining whatsoever. Any ideas...? 

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