Adherent cell line to be used as target cells in a Natural Killer assay?

Hello, I am planning to perform a Natural Killer assay. My target cells are adherent cells so I would like to use an adherent cell line as target cells and positive control rather than using the...

07 August 2016 9,274 0 View

Can someone suggest a pig splenocyte isolation protocol?

17 July 2015 6,157 3 View

Can someone suggest a pig splenocyte isolation protocol?

06 July 2015 2,627 4 View

Does anyone have experience with Alzet Osmotic pumps for L-NIO delivery in mice?

Hello, I would like to use one a osmotic pumps (from Alzet) to delivery L-NIO in mice. There are many types of osmotic pumps and I don't know which one fits better to my experiment. The experiment...

01 February 2015 8,443 4 View

Does anyone know of any Anti-Pig Endothelial Abs?

Does anyone know Endothelial antibodies (like CD31, Von Willebrand, VEGFR or VE-Cadherin) that work well in pigs? Thank you very much in adavance for your help Iker

23 June 2014 1,279 3 View

Does anyone know of any Anti-Pig Endothelial Abs?

Does anyone know Endothelial antibodies (like CD31, Von Willebrand, VEGFR or VE-Cadherin) that work well in pigs? Thank you very much in adavance for your help Iker

05 June 2014 1,979 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View