Adherent cell line to be used as target cells in a Natural Killer assay?

Hello, I am planning to perform a Natural Killer assay. My target cells are adherent cells so I would like to use an adherent cell line as target cells and positive control rather than using the...

07 August 2016 9,274 0 View

Can someone suggest a pig splenocyte isolation protocol?

17 July 2015 6,157 3 View

Can someone suggest a pig splenocyte isolation protocol?

06 July 2015 2,627 4 View

Does anyone have experience with Alzet Osmotic pumps for L-NIO delivery in mice?

Hello, I would like to use one a osmotic pumps (from Alzet) to delivery L-NIO in mice. There are many types of osmotic pumps and I don't know which one fits better to my experiment. The experiment...

01 February 2015 8,443 4 View

Does anyone know of any Anti-Pig Endothelial Abs?

Does anyone know Endothelial antibodies (like CD31, Von Willebrand, VEGFR or VE-Cadherin) that work well in pigs? Thank you very much in adavance for your help Iker

23 June 2014 1,279 3 View

Does anyone have experience doing sections and staining with CorMatrix membranes?

Hello, I am working with the CorMatrix membrane and I looking for some advice as I am new to this field. I would like to include the CorMatrix in OCT and do some sections and staining. Has anyone...

02 March 2014 7,214 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View