Does anyone know Endothelial antibodies (like CD31, Von Willebrand, VEGFR or VE-Cadherin) that work well in pigs?
Thank you very much in adavance for your help
Iker
Hi Iker,
Linscott's directory has a list of Abs against pig CD31:
http://linscottsdirectory.com/search/antibodies/searchStr:cd31/searchMode:specificity/hostId:/reactiveId:Pig,porcine,swine/applicationId:/formatId:/antibodyTypeId:/isoTypeId:/pageSize:100
vWf:
http://linscottsdirectory.com/search/antibodies/searchStr:von%20willebrand/searchMode:specificity/reactiveId:Pig,porcine,swine/pageSize:100
VEGFR:
http://linscottsdirectory.com/search/antibodies/searchStr:VEGFR/searchMode:specificity/reactiveId:Pig,porcine,swine/pageSize:50
and VE-cadherin:
http://linscottsdirectory.com/search/antibodies/searchStr:VE%20cadherin/searchMode:specificity/hostId:/reactiveId:Pig,porcine,swine/applicationId:/formatId:/antibodyTypeId:/isoTypeId:/pageSize:25
Check whether these Abs have been used in publications
Thank you very much for you answer. The information is really useful for me. Really appreciate it.
All the best,
If you have antibodies for ECs of other species, first try them on a positive control porcine sample, as they often cross-react.
Regards, Deon
Thank you very much to all for your answers. I really appreciate the information.
Best wishes
Hello, I am planning to perform a cytotoxic assay with porcine splenocytes as effector cells. I have tried to find the proper concentration of porcine IL-2 that I should use for NKs stimulation...
05 December 2016 5,974 5 View
Hello, I am planning to perform a Natural Killer assay. My target cells are adherent cells so I would like to use an adherent cell line as target cells and positive control rather than using the...
07 August 2016 9,364 0 View
Hello, I need to isolate splenocytes from pig spleens and I have some doubts about the protocol that I hope some can clarify me: 1. Red blood lysis buffer --> I have read that the...
17 July 2015 6,229 3 View
06 July 2015 2,699 4 View
Hello, I would like to use one a osmotic pumps (from Alzet) to delivery L-NIO in mice. There are many types of osmotic pumps and I don't know which one fits better to my experiment. The experiment...
01 February 2015 8,517 4 View
Dear all, I am planning to do a immune response study in pigs and I have a doubt that I hope someone can clarify me: I need to get blood from the pigs and then extract serum and PBMCs (Peripheral...
26 January 2015 7,429 5 View
Does anyone know Endothelial antibodies (like CD31, Von Willebrand, VEGFR or VE-Cadherin) that work well in pigs? Thank you very much in adavance for your help Iker
23 June 2014 1,349 3 View
Hello, I am working with the CorMatrix membrane and I looking for some advice as I am new to this field. I would like to include the CorMatrix in OCT and do some sections and staining. Has anyone...
02 March 2014 7,281 0 View
Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.
09 August 2024 5,605 2 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View