Does anyone have DAPI protocol for staining tissue sections (not cell or bacteria) like fish gonads, liver etc???
I am trying to convert all the intercultural operations of rice cultivation to energy but I do not know how to measure farm machinery in Kg. Also, one article wrote diesel as another component,...
21 June 2023 2,859 0 View
Modèle théorique sur l'impact de la territorialisation de la formation professionnelle sur les stagiaires?
01 April 2023 9,944 2 View
I used two rice variety in seedbed for raising seedling but unfortunately seedling condition is not well enough. In some areas yellowish seedlings were found. So how can I accelerate or enhance...
11 January 2017 8,381 11 View
I am analyzing effect of stress in hub by applying pressure on the keyway surface.I am using Ansys software for this analysis.
05 June 2016 5,435 4 View
I am doing a research on feasibility of storm water management for fire fighting works in school and colleges. I have got the rainfall data but it is in cm per day and mm per three hours. I am...
25 January 2016 9,816 19 View
I want to design a overhead water tank mainly for firefighting purpose. The water source will be storm water (rain water harvest). while designing the water storage tank what methods and equations...
13 October 2015 3,238 3 View
Currently dengue fever is a great threat for Banhladeshi specially for the city dwellers. Hospital has not enough space for their treatmemt and everyday few people are died already. So need your...
01 January 1970 5,933 17 View
Sometime it is being very confusing about on farm, off farm and non farm income. So that I need clarification regardingvthose term with some good example. Please make it clear. Thanks in advance.
01 January 1970 2,171 15 View
Recently organic food is getting much priority in respect of various matters.So that I want to fo some research inbrice field. But I do not have enough idea regarding organic production of rice....
01 January 1970 1,633 1 View
It s very alarming issue for us that day by day OM is decreasing and no management practices are done for improvement. So that I want to know the procedure that how can we improve the OM content...
01 January 1970 1,395 84 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...
05 August 2024 853 3 View
Is this circle one of the rings?
03 August 2024 5,988 0 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
In the aquaculture and fish nutrition research field, a number of growth and somatic indexes are often used, including specific growth rate (SGR), feed conversion rate (FCR), protein efficiency...
22 July 2024 2,480 4 View
Chemical Engineering /Food Engineering
20 July 2024 4,152 4 View
To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...
18 July 2024 9,177 2 View
I suspect my cells are contaminated with mycoplasma. I fixed the cells with 4% PFA and stained them with DAPI. Below is the image I obtained. I don't observe the typical small, rounded DAPI foci...
11 July 2024 7,786 3 View
the molecule name is citated in old literature about organic sunscreen molecules
10 July 2024 5,576 2 View