Hi, I am working with Cardiomyocytes in 3D (differentiated from hIPSC) from FujiFilm Cellular Dynamics. The are very nice to work with but we are testing a highthroughput microfluidic device and are currently using large volumes of very expensive media (from the company). I want to use my own formulation just for maintenance. I will use the recommended media for spheroid formation etc...
There are many options in the literature for this media however I am thinking of using RPMI 1640, 2% B27 supplement & 1%Pen/strep. This will be used for 3 days - 14 days.
Any suggestions/comments on this formulation?
You can use stempromedia 34 supplemented with ascorbic acid , glutamax and transferin. This is expensive option but 3D constructs more than 6 months please check cell zhao et al 2019
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