Finola Cliffe

9 Questions 1 Answers 0 Followers

Questions related from Finola Cliffe

Can someone give me some clarification re. induction of CYP3A4 and concurrent metabolism experiment?

Hi, our lab has recently started working with primary hepatocytes in 3D form. The lot we are using has high basal activity which declines as the spheroids age. We are looking to undertake drug...

22 May 2024 1,377 0 View

Does anyone have any recommendations for maintenance media for 3D cardiomyoctes (from hiPSC)?

Hi, I am working with Cardiomyocytes in 3D (differentiated from hIPSC) from FujiFilm Cellular Dynamics. The are very nice to work with but we are testing a highthroughput microfluidic device and...

21 November 2023 6,152 2 View

Has anyone used Life Technologies Vivid CYP screening kits with 3D spheroids/organoids?

Has anyone successfully used Life Technologies Vivid CYP screening kits with 3D spheroids/organoids? I am finding it difficult to find anything in the literature. Ideally I'd like to compare CYP...

23 January 2023 3,297 0 View

Can anyone help me with an immunostaining protocol for HepG2 spheroids?

Hi, I have used the following protocol for HepG2 spheroid immunostaining, however the E-Cadherin staining is very weak and difficult to see. I am using an Olympus IX70 microscope to image. I...

25 January 2022 7,295 4 View

How do you bond PEGDA to PMMA?

Hi, I want to create a layer of PEGDA onto a U-shaped PMMA surface. The hyrogel formation works well however the PEGDA layer once set is moving within the PMMA wells as it is not bonded to the...

22 September 2021 8,050 1 View

Could anyone give recommendations for a fluorescent microscope for spheroid/organoid imaging?

Hi, I am looking to purchase a fluorescent microscope for imaging spheroids/organoids stained with live/dead dyes. Would anyone have a user recommendation for such a microscope? I have a...

15 June 2020 3,882 0 View

Is it possible to successfully cryopreserve mammalian cells in Liq N after cooling in minus 20 freezer?

Hi, can anyone tell me if it is possible to successfully store mammalian cell lines that have been cooled in a minus 20 mechanical freezer before storage in liquid nitrogen. I do not have access...

20 August 2019 1,452 0 View

How quickly is drug such as Doxorubicin taken up by individual cells?

Can anyone tell me how quickly individual cells can take up a drug resulting in an effect? I see alot in the literature where cells are incubated with drugs for up to 24 hours but no specific...

06 February 2019 746 3 View

How long does it take for E. coli to infect mammalian cells in vitro?

Hi, I am doing some studies with effect of antibiotics on E.coli after infection of Mammalian cells. I'm specifically looking at MCF-7 cell lines. Could anyone give me some insight into how long...

18 December 2014 1,452 1 View