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Questions related from Finola Cliffe
Hi, our lab has recently started working with primary hepatocytes in 3D form. The lot we are using has high basal activity which declines as the spheroids age. We are looking to undertake drug...
22 May 2024 1,298 0 View
Hi, I am working with Cardiomyocytes in 3D (differentiated from hIPSC) from FujiFilm Cellular Dynamics. The are very nice to work with but we are testing a highthroughput microfluidic device and...
21 November 2023 6,104 2 View
Has anyone successfully used Life Technologies Vivid CYP screening kits with 3D spheroids/organoids? I am finding it difficult to find anything in the literature. Ideally I'd like to compare CYP...
23 January 2023 3,257 0 View
Hi, I have used the following protocol for HepG2 spheroid immunostaining, however the E-Cadherin staining is very weak and difficult to see. I am using an Olympus IX70 microscope to image. I...
25 January 2022 7,257 4 View
Hi, I want to create a layer of PEGDA onto a U-shaped PMMA surface. The hyrogel formation works well however the PEGDA layer once set is moving within the PMMA wells as it is not bonded to the...
22 September 2021 8,013 1 View
Hi, I am looking to purchase a fluorescent microscope for imaging spheroids/organoids stained with live/dead dyes. Would anyone have a user recommendation for such a microscope? I have a...
15 June 2020 3,844 0 View
Can anyone tell me how quickly individual cells can take up a drug resulting in an effect? I see alot in the literature where cells are incubated with drugs for up to 24 hours but no specific...
06 February 2019 707 3 View
Hi, I am doing some studies with effect of antibiotics on E.coli after infection of Mammalian cells. I'm specifically looking at MCF-7 cell lines. Could anyone give me some insight into how long...
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