Browning is a common problem in my explants too, I use polyvinylpyrrolidone (PVP) prior to inoculation to prevent browning. Initial washing with bavistin may reduce contamination. Juvenile explants show less browning and contamination than of mature explants.

Firstly use juvenile explants for culture. You can also use antioxidants like- PVP, Ascorbic acid, Activated Charcoal. Frequent subculture of explants on to the fresh culture media and putting them in dark and at low temperature can be effective for successful establishment of plants in vitro and encountering phenolic browning.

Thank you both for your answers, i am applying some of your recomendations, let us see if it works. Best regards

Hola el polivinilpirrolidona (PVP) yodada me resulto en los explantes de platáno en la etapa de esterilizacón y luego pasarlos por 10 min una vez esterilizados los explantes en una solución de ácido ascorbico de 1mg/ 200ml de agua destilada esteril. Que te resulte!!!!

PVP and ascorbic acid are good antioxidant agents for browning effect. If you intend to use activated charcoal, you might need to optimise its concentration. High concentration of activated charcoal might cause negative effect for plant growth and absorb the growth regulators used. You may also do frequent subculture.

Dear Zabala

You can use activated charcoal by 2%  to the media or reculture every three days until they get rid of the browning

good luck

Browning can be limited or avoided if the explants are maintained in immersion inside  a solution enriched of antioxydants (citric or ascorbic acid) before the sterilization and/or adding PVP or reduced gluthation to the agarised medium

Hello

I think preparation of the explants in the spring, when the shoots are newly emerged, but they are tight, and the incubation of the cultures at 4 °C for 5 days can well prevent browning of the cultures.