I am trying to sensitize a B6 mouse against the Ovalbumin protein, so I was going to inject the protein mixed with some sort of adjuvant (Freund's most likely). Does anybody have experience with this? I would like to know what concentration of protein to use, how much adjuvant to use, what the best adjuvant is, how many times to inject, route of administration (IV, IP, sub-cutaneous, etc.) length of time before sensitization, and any other variables to consider? Any information or insight to this would be very greatly appreciated.
I presume that you want to sensitize mice to delayed type hypersensitivity (DTH) to ovalbumin. You could do this by IV injection of BCG about 7 - 10 days before injection of ovalbumin. There are other stimulators of DTH, including tubercle bacillus, which is part of Freund's complete adjuvant, pertussis toxin, etc. If you want to stimulate mice to IgE type hypersensitivity, you need to choose an adjuvant that stimulates Th2 type response. Aluminum containing adjuvants and proteins from parasites stimulate Th2 type response.
Dear Josh,
I used balb/c mice for my research. I injected the ova (50ug) + alum (1mg) as an adjuvant via i.p. on day 1,7 and 14. The link below may help you as it show step by step on how to prepare ova.
http://www.bio-protocol.org/wenzhang.aspx?id=644
- Badges
- Science method
- Similar topics
- Mathematics
- Statistics
- Statistical Modeling
- Analyses of Variance
- ANOVA