Salme Suhana

6 Questions 10 Answers 0 Followers

Questions related from Salme Suhana

How to measure pH of ice sample?

Hi, anyone can share how to check the pH of ice sample? Need to wait for the ice to melt to room temperature (25°C)?

24 February 2022 2,457 2 View

Is anyone familiar with using multiplex assay for cytokine detection?

Hi,Is there anyone familiar with multiplex assay for detecting cytokine such as IL-4, IL-5 and IFN-y. My sample is bronchoalveolar lavage fluid and I plan to use luminex screening assay from...

09 December 2014 7,471 13 View

What is the correct technique to obtain bronchoalveolar lavage fluid (BALF) for an asthmatic study?

Is there anyone that can share the correct way to obtain BALF from mice models of asthma? I've tried to lavage the lung via trachea with phosphate buffered saline (PBS) however after I inserted...

11 April 2014 9,393 3 View

Do you have experience culturing the hRBL-2H3 cell line?

I'm going to use the RBL-2H3 cell line for my in vitro study involving the inhibitory activity of honey bee product on mast cell degranulation. I just want to know if there is anyone who has...

28 January 2014 7,676 4 View

Can anyone help with calculating total phenolic content (TPC) and total flavonoid content (TFC)?

Can anyone explain what is the formula and how to calculate TPC and TFC? I would be glad if someone can provide me a link to research journal regarding this matter since most of the journals that...

03 January 2014 5,387 25 View

Developing asthma in mice

How long does it take to sensitize mice with ovalbumin so that they develop acute and chronic phase of asthma or allergic airway inflammation?

14 March 2013 2,812 3 View