Does anyone have any experience with BrU-labelled RNA IPs?

11 February 2016 1 7K Report

I am currently metabolically labelling nascent RNA, however I am having problems eluting the BrU-labelled RNA from the beads. I am heating the beads to 95C for 10 mins. Also I am having trouble obtaining a clean IP in my negative non-labelled samples.

Any suggestions about RNA IPs would be much appreciated.

Thanks

Nikolay Rozhkov

if using anti-BrU agarose beads (santa cruz) use elution buffer (5 mM Tris–HCl, pH 7.5, 300 mM NaCl,

1 mM EDTA, 20 mM DTT, and 0.1 % SDS). 3-4 elutions with small volume (125-150ul) will wash off the labeled RNA.

good luck

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