Does anyone have any experience with blood collected in PAXgene tubes for ELISA?

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What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

What are the parameters that plays a role in life-time degradation models of concrete? what are the most important tests to measure these parameters?

I'm working on a research about the prediction life-time models for concrete in harsh environment. I have to answer those two questions.

14 November 2020 2,043 7 View

Does anyone know of historic sites that have artist in residency programs?

A research assistant and I are conducting primary research on artist in residency programs (artists, writers, etc.) and the use of contemporary art at historic sites to reinvigorate or...

27 September 2020 9,355 4 View

What is PROSPERO - University of York regarding Systematic Literature Reviews?

I have seen for the first time a systematic literature review and meta-analysis that has prospectively registered their review in PROSPERO and I am wondering what is the main reason for creating...

04 August 2020 4,167 4 View

What causes the copy detection limitation in PCR?

Hi all, I wish to understand why doesn't all polymerase can detect a single copy of region of interest? While some polymerase is more sensitive (like dreamtaq), and some are less sensitive, what...

02 June 2020 4,397 21 View

Evidence-based resources for helping kids cope with debilitating & likely fatal disease?

A colleague asked me for any evidence-based approaches to helping kids (and parents) cope with a life-threatening disease, Duchene muscular dystrophy. The child doesn't know he is sick yet. Does...

24 March 2020 2,046 3 View

Does anyone has experience with NEB "time-saver enzyme" overnight digestion?

Hi all, I used the Thermo-ANZA series for overnight digestion of plasmid backbone to ensure clean digestion without any problem. How about NEB? The website said all NEB "time-saver enzyme"...

24 March 2020 7,636 2 View

Difference between cell culture plates and flasks?

In the lab we always use culture plates (33, 60, 100, 150mm) for passing cells and conducting all sorts of experiments. I wonder what is the perks of using cell culture plates v.s. flasks? Should...

12 March 2020 1,361 2 View

How can can I find a none fee charging credible journal to publish a co authored paper?

I am looking for a none fee charging journal to publish a coathored paper. Its in special education

20 January 2020 5,203 3 View

Can I transfer Spheroid to normal attachment plates for other assays? How will it affect the morphology and consistency?

Do you guys have any experience in transferring spheroid produced in low attachment plate to a high attachment plate after spheroid production? Would it affect the morphology of the spheroid? I...

11 December 2019 1,918 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View