I'm looking for a method to detect coenzyme Q10 in food (dairy products), using normal phase HPLC. Can anyone help me?
Analysis of phospho- and sphingolipids in dairy products by a new HPLC method
Roeland Rombaut, John Van Camp UGent and Koen Dewettinck UGent
(2005) JOURNAL OF DAIRY SCIENCE. 88(2). p.482-488
Effect of Micronutrient-Enriched Sunflower Oils on Plasma Lipid Profile and Antioxidant Status in High-Fat-Fed Rats
R. Di Benedetto, L. Attorri, F. Chiarotti, A. Eusepi, A. Di Biase and S. Salvati
J. Agric. Food Chem., 2010, 58 (9), pp 5328–5333
Publication Date (Web): March 31, 2010 (Article)
DOI: 10.1021/jf904360y Tocopherols and sterols were determined by ANIA (Valencia, Spain); the former were quantified by high-performance liquid chromatography coupled to photodiode array detection (HPLC/DAD), using the official method NF ISO 9936, while the latter were quantified by gas chromatography using the methodology based on the EN ISO 12228:1999 standard. ... A new sensitive and selective method has been developed for the quantification of the total coenzyme Q9 (CoQ9) and coenzyme Q10 (CoQ10) concn. in vegetable oil samples. ... To validate the proposed method, anal. of the only BCR Ref. Material available, with a certified content of α-tocopherol (margarine CRM 122), was carried out using the automated methodol., the results found being in agreement with the certified value. ...
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