I want to use ex vivo fibrin taken from a skin wound and explore its degradation by fibroblast in vitro...But I have no idea about using this kind of matrix...
Hi, I would like to export the FLR data acquired via MassLynx 4.1 software to Empower for processing. Unfortunately, FLR data are recorded as Analog data and therefore cannot be converted to...
01 July 2020 7,018 0 View
I need to have a proof that the cells in culture are indeed fibroblasts
20 June 2013 8,531 5 View
Where and when was the 1st PMHNP program administered?
01 January 1970 2,658 2 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Machine Learning for Automated Diagnosis of Skin Cancer with Dermoscopy Images
21 July 2024 5,471 0 View
Hi everybody, I wonder is there a way to prepare foam from iso-propanol/water mixture? The content of this alcohol should be 70 – 80 w.%. I plan to use silicone-based surfactants, I have several...
15 July 2024 3,539 0 View
Dear all, We've been doing transesterification with our raw materials - a mixture of pairing oil (which is said to came from the skin part of a coconut) and crude coconut oil. During the addition...
14 July 2024 2,200 0 View
Hello, I isolated total histones successfully from the skin primary cells by acid extraction. I wanted to detect histone H1.2 expression in different conditions by western blot. Can some one...
10 July 2024 9,165 1 View
I have hemostatic nanofibers of hydrophilic nature. While fibrin formation from fibrinogen can be qualitatively visualized using SEM. What assays can be performed on nanofibers to quantify the...
08 July 2024 3,714 1 View
Occasionally we observe lack of staining in the inner part of a skin section processed with indirect immunofluorescence. Can be due to incomplete removal of the cryoprotectan during the wash? Any...
07 July 2024 8,200 2 View
I am using fibroblast growth factor, purchased in powder form. What suitable substance should be added to get it in a liquid form? Thanks
03 July 2024 9,866 2 View
Hello, I have been performing Western Blots to study the protein levels of a rodent skin and muscle sample. However, I have not been able to produce homogenous reference bands. I suspect that...
26 June 2024 5,913 3 View