Does anybody have an idea how to characterize fibroblasts (from dermis or other tissue) by FACS?

More Gwenaël Rolin's questions See All

How to export FLR data from MassLynx to Empower?

Hi, I would like to export the FLR data acquired via MassLynx 4.1 software to Empower for processing. Unfortunately, FLR data are recorded as Analog data and therefore cannot be converted to...

01 July 2020 7,018 0 View

Psychiatric Mental Health Nurse Practitioner program.

Where and when was the 1st PMHNP program administered?

01 January 1970 2,658 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Karl Enevold

presence of cytochalsin inhibitable (?) and masoprocal potentiation of c14 glucose uptake of Glut-1, is an easy assay.

Gwenaël Rolin

Dear Karl, thx for your answer! you said that this assay is easy, do you mean that it exist a kit for that? or could you please explain me how does it work?

It uses C-14 glucose to measure cellular uptake via glut-1

Ok, but is it specific only for fibroblasts?

If you can send me a link to an article dealing with this method...