I need to have a proof that the cells in culture are indeed fibroblasts
presence of cytochalsin inhibitable (?) and masoprocal potentiation of c14 glucose uptake of Glut-1, is an easy assay.
Dear Karl, thx for your answer! you said that this assay is easy, do you mean that it exist a kit for that? or could you please explain me how does it work?
It uses C-14 glucose to measure cellular uptake via glut-1
Ok, but is it specific only for fibroblasts?
If you can send me a link to an article dealing with this method...
Where and when was the 1st PMHNP program administered?
01 January 1970 2,430 2 View
02 March 2021 3,060 3 View
28 February 2021 9,936 2 View
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines
24 February 2021 2,268 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
22 February 2021 9,932 11 View