Does anyone have any experience in ITK-SNAP for muscle DTI analysis and fiber tracking?

More Ezgi Kirimli's questions See All

I am obtaining low qPCR efficiency for absolute quantification which is cause by higher Ct jump after 10^6 dilution.What could be the reason for that?

There is a jump in the expected Ct change when I go from dilution 10^6 to 10^5. When I prepare my standard curve, the most diluted standards have a difference of around 3 cycles, that would be...

05 September 2023 4,675 0 View

Why do 3t3-l1 cells peel off without differentiation?

Hello, When preadipocyte cells were seeded to differentiate and confluent 3 days later, detachment and peeling occurred in all wells when changing the culture medium. 60,000 for 12 wells. I am...

23 July 2023 5,967 3 View

Can the transcription factor activity assay method be used instead of western for PPAR and CEBP?

I m investigating sirtuin1 and sirtuin 2 gene expression in the 3T3-L1 preadipocyte cell line. What would you recommend for protein analysis instead of western? thank you,

01 January 2022 7,880 3 View

What do you think about measuring activity with Sirtuin activity assay, which is a fluorometric method, instead of western blotting?

I m investigating sirtuin1 and sirtuin 2 gene expression in the 3T3-L1 preadipocyte cell line. What would you recommend for protein analysis instead of western? thank you, Ezgi

01 January 2022 598 1 View

Have you ever experience Beta actin to be detected on different range than it supposed to be (which is 43 kDa) in western blot?

Edit: image uploaded I've blotted for beta-actin on cell culture protein lysates many times but the beta-actin detection range was not between 37 - 50 kDa range but 25 - 37 kDa range each time....

09 September 2021 4,074 4 View

Dichotomous variable in regression - what if one category has a low percentage?

I have a sample with 138 participants. Only 6 of them reported living alone (%4.3) while the remaining 132 share the household with others (family/partner/housemate etc.) I am trying to decide...

23 July 2021 3,872 6 View

How can I analyze a path analysis with a bidirectional relationship (X<--> C) in Mplus?

This is my input in mplus. When I run the model, the program did not work and give output as usual. It calculated the model but not give the results of specific indirect effects. I also do not...

11 May 2021 9,054 4 View

Right after loading astrocytes with Fura-2 AM, fixing them by PFA and observe the calcium influx would work or not?

I have been working on astrocyte from primary glial cell culture and I want to observe calcium influx after several treatment. I know once Fura-2 AM in the cell, intrinsic esterases hydrolyze the...

18 February 2021 754 3 View

I want to create Amphiphilic Polysaccharide and PLA MPs by electrospray technique (single axial). Could you suggest suitable solvent ?

I know that PLA is soluble in DCM, not soluble in water and hard to dissolve in DMF. What kind of solvents will be suitable for electrospray system for the amphiphilic polysaccharide and...

13 October 2020 3,706 4 View

Does anybody look Erasmus K2 Projects Partnership?

Hello All, We are currently looking for Erasmus K2 Project partner in Europeen countries. Are there anyone interested in becoming a project partner in the social sciences and (or technical...

10 December 2019 6,663 2 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

HAs anyone used TGA to find activation enegy of water evaporation?

I have different polymer solutions and I hypothesize that water molecules are retained based on the water-polymer interactions, so the amount of free water is less to intercat with drug tablet....

28 July 2024 9,545 6 View

Expansion thermal of muscle ?

What is the thermal expansion coefficient of human muscle?

27 July 2024 7,190 0 View

For anyone that works with Human Aortic Smooth Muscle Cells(HASMCs), what seeding density do you use when plating?

I am struggling to find a seeding density that will allow my cells to not aggregate together. Anything helps!

24 July 2024 3,460 1 View

How to get LP fiber modes directly from Comsol?

Hi there, I'm new to modelling with comsol. I would like to ask if it is possible for comsol to output linearly polarized (LP) modes? I tried modelling a simple single mode fiber with an enlarged...

23 July 2024 4,019 3 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Does anyone have sources where I can find the contraction ratios (% shortening of muscle length during full contraction) of specific muscles?

I founde some textbooks stating that typical skeletal muscles can shorten up to 70% (optimally up to 30%) during contraction. However, I would like to look at muscles per region. This might seem...

21 July 2024 399 3 View

Does EMG show muscle spasms?

Electromyography waveforms reflect the electrical activity of a muscle. But does it reveal muscle spasms (transient) or cramps (prolonged spasm)? If so, what does it look like?

20 July 2024 5,951 1 View