Hi,
Does anyone have any recommendations for what sonication conditions are optimal for MCF7 BUS cells? I have tried up to high power for 45 mins, 40 seconds on/off and am still battling to get fragments under 500 bp. My SDS concentration is 1%. I am just slowly increasing the number of cycles and cycle length but was hoping someone might have a miracle answer for me?
Any advice will be greatly appreciated!
Which platform (sonicator) you are using for sonication. Since it is highly depend on the instrument.
You may ask Covaris for their sonication condition if your institute owns this company's sonicator.
Hi Guys,
I managed to figure it out. just extended the sonication time to 60 seconds. Thanks for your assistance.
I have a detailed protocol on optimal sonication conditions for MCF7 using both a multi sonicator and a probe sonicator if you need further assictance.
Johanna Kristina Samuelsson Could you send me that protocol, please? Thanks!
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