Does anyone have an specific protocol for endothelial cell isolation from different tissues or sources?
I have one for human umbilical vein endothelial cells, if you're interested.
Yes I am interested! Thank you!
I've attached our protocol as a PDF. As a note - for the step where you knead the cord, it states 2-3 times; I would normally do ~7. If you have any other questions, I'd be happy to answer them to the best of my ability.
I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...
15 February 2021 655 4 View
Dear all, I've tried many FGFR4 antibodies I saw in the literature but some of them are not useful for IHC and some are only useful for patients slides not for PDX. Do you know a FGFR4 antibody...
14 February 2021 900 0 View
I need to do a mitochondrial ATP quantification assay in insects and for this, I have a bioluminescent ATP assay kit but I don't have a protocol yet. I'd like to know if you have some kind of...
24 January 2021 3,655 3 View
26 December 2020 9,273 6 View
Ethics and moral values have always being an essential part of teaching and scientific research. How do you teach and evaluate these essential subjects in your daily interaction with students?
23 December 2020 5,058 7 View
I'm aware that in order to perform any vegetation index like NDVI, SAVI or OSAVI you need to do some atmospheric, radiometric and terrain corrections. I'm pretty sure that Level 2 images from...
21 December 2020 4,088 3 View
We are investigating the acceptance of jellyfish-based food dishes in Latin America. If you are interested in the topic, please fill out the anonymous survey at the following link. Thanks in...
06 December 2020 4,266 2 View
I am looking for a good Hoagland nutrients solution protocol modified for alfalfa (stock and working). I will use it for hydroponic cultivation of alfalfa. I am not satisfied with one I have. I'll...
25 November 2020 1,791 1 View
we also want this composite material to be able to be modeled for mechanical testing.
19 November 2020 2,069 4 View
Which is the best DNAse (or DNAse kit) you have worked with? I am looking for a good DNAse that can remove DNA from an RNA sample in one pass. Material would be used for future RT-qPCR analysis....
18 November 2020 8,769 3 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...
03 March 2021 1,931 5 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
02 March 2021 3,060 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...
01 March 2021 9,332 6 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View