Does anyone have an specific protocol for endothelial cell isolation?

More Marta Garcia-Contreras's questions See All

Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View

Paired t-test or unpaired t-test for my quantitative data with SPSS?

I am conducting a qualitative-driven approach to mixed-method research. The role of my quantitative data is to corroborate the findings of the qualitative data. Qualitative data has been collected...

24 July 2024 9,799 3 View

How is the preparation to obtain secondary metabolic extracts?

Hi everyone! I am working with bacteria and fungal collected from marine sediments. I would to know the methodology since the preparation of biomass until the extraction and stored of secondary...

03 July 2024 6,177 3 View

C3D file, how to extract a vídeo file from it?

I made a series of jump tests in subjects, everything was recorded with Vicon. I have data from markers and force plate. We recorded the tests before and after an intervention. My question is, I...

23 June 2024 9,381 4 View

Who is the use of Generative Artificial Intelligence in Qualitative Research?

One of the most intriguing frontiers in contemporary research is the application of Generative Artificial Intelligence (GenAI) in qualitative research. This technological advance offers tools that...

13 June 2024 781 2 View

Is there a plugin for weka to read *.sav files from SPSS?

Hello there. Several references say that there is a reader for .sav files from SPSS in Weka, but I just can´t find it. Can anyone provide help? Thanks in advance

12 June 2024 3,844 2 View

Which term is preferable between Fictional Language, Fictitious Language, Artificial Language, or Constructed Language in fiction?

The terms Fictional Language, Fictitious Language, Artificial Language, or Constructed Language in fiction has been used interchangeably in papers that I have read. Are there differences in these...

05 June 2024 3,453 7 View

Can you use the diluted plasma from the Ficoll gradient separation technique for further experiments?

I was wondering if the plasma that is in the top layer after centrifugation can be used to assess proteins, metabolites, hormones, or other molecules, or if the ingredients of the Ficoll alter the...

03 June 2024 1,852 4 View

Have you combined pragmatism and interpretivism before?

Has anyone combined pragmatism and interpretivism in their research? If so, could you please recommend some reading to help with this? I am currently writing the methodological chapter for my PhD...

21 May 2024 2,639 2 View

How to do an APA Format Table of a Repeated Measure MANOVA?

I've have been digging on the internet and I could only find one-way ANOVA APA format table example.

15 May 2024 9,390 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

DeAnna C. Bublitz

I have one for human umbilical vein endothelial cells, if you're interested.

Marta Garcia-Contreras

Yes I am interested! Thank you!

I've attached our protocol as a PDF. As a note - for the step where you knead the cord, it states 2-3 times; I would normally do ~7. If you have any other questions, I'd be happy to answer them to the best of my ability.