Pawan Singh

2 Questions 12 Answers 0 Followers

Questions related from Pawan Singh

How do I make a very concentrated HeLa cell lysate without membrane or lipid for a western blot?

I have around 100ul of HeLa cells pellet. I want to make a very concentrated lysate in around 100ul of vol for westren blot. I want all the proteins (membrane + soluble) but not any lipid or...

12 October 2015 2,496 4 View

Any suggestion for fluorescent lysosomal cargo for co-localization with DQ-BSA in HeLa cells for confocal Microscopy?

I want to analyse lysosomal trafficking of a WT bacteria and some KO bacteria in HeLa cells with respect to DQ-BSA red. I want to use a fluorescent cargo as a +ve control for my experiment for...

23 September 2014 6,855 7 View