Does anyone have advice on ATCC strain shipping?

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My question concerns MTT cell viability assay?

Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...

21 July 2024 9,914 1 View

Guidance needed for preparing the hydrogel samples for the XRD instrument?

I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...

20 July 2024 3,611 4 View

How can I extract the mathematical equation from existing Neural Network Model?

There exists a neural network model designed to predict a specific output, detailed in a published article. The model comprises 14 inputs, each normalized with minimum and maximum parameters...

14 July 2024 2,714 3 View

Why does my IVT gRNA product gives HMW smear in gel?

We are using MEGAshortscript™ T7 Transcription Kit for transcribing gRNAs from linearized plasmid templates. (MSP3485 digested with HindIII) (https://www.addgene.org/140082/) We let the reaction...

01 July 2024 6,342 0 View

How can Abaqus CAE be utilized to assess the variation in wall thickness throughout a plastic bottle following a blow molding simulation?

Hello ResearchGate Community, How can Abaqus CAE be utilized to assess the variation in wall thickness throughout a plastic bottle following a blow molding simulation?

26 June 2024 9,281 4 View

JCR 2024 Impact Factor Download File-Factors caused decline in IF are?

Why the IF of almost all journals declined so much? For the file download go to https://www.researchgate.net/post/JCR_Impact_Factor_2024-Factors_causes_decline_in_IF

21 June 2024 3,358 0 View

When use a HOMER Pro to design hybrid system PV/electrolyzer/hydrogen tank. How can I use all electrical load to power the electrolyzer?

Simulating a Hybrid System with PV, Grid, Electrolyzer (No Load) in HOMER. here is a question about how to use HOMER Pro to design a solar PV system for water electrolysis using electrolyzer for...

05 June 2024 5,609 0 View

Expert questionnaire, Is it possible for people with a specialty in music psychology to evaluate this questionnaire? Through the link below?

It is possible for people specialized in music psychology to evaluate this questionnaire Through the link below Thank you https://forms.gle/qzoD7jN2UDUhMjwQ7

02 June 2024 9,546 0 View

How can we extract DNA from yeast isolates without using any lysis buffer or lysis enzymes? Is there any manual protocol?

31 May 2024 548 4 View

Please could colect all my publications under my name ?

please could collect all my publications under my name ?

30 May 2024 2,891 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How to have sustainable development for the artic region?

How can we have both environmental and indigenous people protection together with shipping all year-round, as well as natural resources exploitation?

18 July 2024 1,077 3 View

Is bacteriological activity in deep sandy groundwater proportional to the nitrate level knowing that O2 is limited?

The recharge of deep sandy groundwater is slow. Does the high level of nitrate promote bacteriological activity knowing that it requires a high level of O2? Thanks

10 July 2024 9,883 5 View

How to obtain authoritative data on the international shipping industry?

It is best to have the route distance between ports, the size of the shipping alliance market, direct service, the number of ships owned by the shipping alliance and the congestion of ships in the...

06 July 2024 845 1 View

Where can I download Bergey's Manual of Determinative Bacteriology?

27 June 2024 8,055 2 View

Hello I would appreciate it if someone could guide me on how to get the input and output pressure in the compressor?

I would be grateful if someone could guide me how to get the input and output pressure in the compressor used in solar heat pumps? For example, in this article Annual comparative performance of...

12 June 2024 8,985 5 View

Maritime economics focuses on the economic principles?

Maritime economics focuses on the economic principles and dynamics governing the maritime industry. This field encompasses various aspects, such as the functioning of shipping markets, port...

08 June 2024 3,434 1 View

The gap in diagnosing intra-thoracic tuberculosis in children is not closed despite the advanncement in microbiology fields?

In 2024, childhood tuberculosis will still be a significant global public health issue, especially in underdeveloped nations (LMICS). It is true that the underestimation of the disease's extent...

31 May 2024 5,221 0 View

Active Collaboration in Research ?

Dear LinkedIn Family, Greetings of the day from Pakistan. I am Actively seeking for Collaboration in Research, Training programs students/faculty Exchange program globally. Area of Interest...

03 April 2024 3,312 1 View

Compressor used in heat pumps size needed?

I have modelled a heat pump and for a cooling capacity of 5 to 10kw, what would be the displacement volume, piston diameter and stroke length, number of cylinder in the compressor. I tried to...

03 April 2024 10,002 2 View