13 Questions 24 Answers 0 Followers
Questions related from İbrahim Türkel
Hi My question is, is it acceptable or valid to detect plasmid mediated antimicrobial resistance genes via Rt-Qpcr. İs there any limitaitons to isolate RNA from plasmid and calculate gene expression?
22 January 2017 1,370 4 View
Since getting data from my real time pcr experiments, i'm struggling to analyze these datas. i have 100 bacteria strain isolated from different patients and i want to detect gene expression...
11 April 2016 5,666 10 View
Hi all We identified some lactic acid bacteria as lactococcus lactis ssp lactis, lactococcus lactis spp. cremoris and enterococcus faecium with API strips. But we have some problem about molecular...
05 September 2015 6,176 1 View
Hi all i want to measure efflux activity of klebsiella penumoniae with etbr agar cartwell method but i dont know how i must this start this assay. i am rookie as well as :) is the any one use...
29 July 2015 4,532 5 View
Hi all i want to isolate genomic dna for PCR. boling method or fenol-chloro extraction fits for klebsiella, especially gram negative bacteria.
14 July 2015 2,935 7 View
Hi all I am trying to detect beta lactamase by combine disk test with ceftazidime,ceftazidime/clavulanete and cefotaxime, cefotaxime/clavulanate as described at CLSI. I don't see any zone at some...
25 June 2015 1,497 12 View
Question is how can I measure gene expression levels of resistance genes treated with antibiotics?
06 May 2015 7,191 5 View
Hi all. Can i determine the efflux pump expression against efflux pump inhibitors with microarray? How can i isolate bacteria treated with inhibitor?
02 May 2015 6,753 2 View
We first isolate lactic acid bacterias from milk samples on MRS Agar Medium. Then we use M17 agar for lactococus species-specific medium. Can we say these strains lactococcus spp or not without...
29 April 2015 3,526 15 View
I need Klebsiella pneumoniae ATCC 700721(MGH 78578) for my master project. Can anyone provide me this strain because it's too expensive to transfer this strain from ATCC? Best wishes
20 March 2015 6,419 2 View
Hi all, I am working on detecting antibiotic susceptibility of klebsiella pneumoniae with different discs. My question is which solution must be use to determine 0.5 Mcfarland? 0.9% NaCl or...
03 March 2015 5,195 8 View
Can you help what must I do to purify total RNA from klebsiella pneumonaie? I have GeneJet RNA purification kit and it does not include lysozyme. It contains proteinase k. Manufacturer protocol...
23 February 2015 4,616 8 View
i am trying to work on efflux pump systems such ac AcrAB, kexd and others in my Msc project. i cant find enough information about PCR protocols. i am looking for to some help for my project.
18 February 2015 8,003 1 View