İbrahim Türkel

16 Questions 27 Answers 0 Followers

Questions related from İbrahim Türkel

Is it acceptable literally to detect expression level of beta-lactamase gene expression in Rt-qpcr?

Hi My question is, is it acceptable or valid to detect plasmid mediated antimicrobial resistance genes via Rt-Qpcr. İs there any limitaitons to isolate RNA from plasmid and calculate gene expression?

22 January 2017 1,421 4 View

Can i use 2-ΔΔCt and 2-ΔCt formulas together for real time data analyze?

Since getting data from my real time pcr experiments, i'm struggling to analyze these datas. i have 100 bacteria strain isolated from different patients and i want to detect gene expression...

11 April 2016 5,724 10 View

How do I do total protein extraction from klebsiella pneumonia?

To detect difference, I want to extract total protein from klebsiella pneumonia, does anybody have a standart protocol wanna share? Best wishes

17 September 2015 9,727 1 View

Difference of species-spesific primers for molecular typing Lactic Acid Bacteria?

Hi all We identified some lactic acid bacteria as lactococcus lactis ssp lactis, lactococcus lactis spp. cremoris and enterococcus faecium with API strips. But we have some problem about molecular...

05 September 2015 6,230 1 View

Can anyone help with the Et-Br Cartwheel Method?

Hi all i want to measure efflux activity of klebsiella penumoniae with etbr agar cartwell method but i dont know how i must this start this assay. i am rookie as well as :) is the any one use...

29 July 2015 4,579 5 View

Which manual method suitable for dna extraction from Klebsiella pneumoniae?

Hi all i want to isolate genomic dna for PCR. boling method or fenol-chloro extraction fits for klebsiella, especially gram negative bacteria.

14 July 2015 2,989 7 View

How I detect for beta lactamase?

Hi all I am trying to detect beta lactamase by combine disk test with ceftazidime,ceftazidime/clavulanete and cefotaxime, cefotaxime/clavulanate as described at CLSI. I don't see any zone at some...

25 June 2015 1,560 12 View

Can anyone provide insight regarding Staphylococcus aureus efflux pump?

Hi all i want to determine efflux pump nora, norb, norc at s.aureus. which strain i must use at pvr for positive control?

25 June 2015 8,693 3 View

Does anyone know about klebsiella HSP60 (Groel) antibody?

Hi all I will try to detect hsp60-groel heat shock protein at klebsiella pneumoniae with western blot analysis. But i cant find this antibody. so can i use e.coli groel antibody or common anti...

15 May 2015 3,006 1 View

How can I measure gene expression treated with antibiotic?

Question is how can I measure gene expression levels of resistance genes treated with antibiotics?

06 May 2015 7,241 5 View

Can anyone help with measuring Efflux Pump İnhibitors effect on efflux pump expression on microarray?

Hi all. Can i determine the efflux pump expression against efflux pump inhibitors with microarray? How can i isolate bacteria treated with inhibitor?

02 May 2015 6,811 2 View

Does anyone know about lactococcus strain isolation from milk?

We first isolate lactic acid bacterias from milk samples on MRS Agar Medium. Then we use M17 agar for lactococus species-specific medium. Can we say these strains lactococcus spp or not without...

29 April 2015 3,582 15 View

Does anyone have advice on ATCC strain shipping?

I need Klebsiella pneumoniae ATCC 700721(MGH 78578) for my master project. Can anyone provide me this strain because it's too expensive to transfer this strain from ATCC? Best wishes

20 March 2015 6,471 2 View

Is anyone familiar with the Kirby-Bauer Disk Diffusion Method?

Hi all, I am working on detecting antibiotic susceptibility of klebsiella pneumoniae with different discs. My question is which solution must be use to determine 0.5 Mcfarland? 0.9% NaCl or...

03 March 2015 5,250 8 View

Can I use Proteinase K instead of lysozyme?

Can you help what must I do to purify total RNA from klebsiella pneumonaie? I have GeneJet RNA purification kit and it does not include lysozyme. It contains proteinase k. Manufacturer protocol...

23 February 2015 4,672 8 View

Is anyone working on an efflux pump systems in klebsiella pneumoniae?

i am trying to work on efflux pump systems such ac AcrAB, kexd and others in my Msc project. i cant find enough information about PCR protocols. i am looking for to some help for my project.

18 February 2015 8,056 1 View