How to calculate the degradation rate in percentage ?

I performed the BCA assay to calculate protein content over the time period. However, I'm not understanding how I can calculate the degradation rate in percentage at each time point? What formula...

10 January 2021 9,094 3 View

Why peaks appear down in case of absorbance measurement in FTIR?

I tested the Titanium disc in FTIR after treating it with chemicals. But the absorbance peaks are showing down. I don't know what is wrong going on.

07 November 2019 1,963 5 View

I need to extract total RNA from protoplasts, could anyone help me with a detailed protocol?

22 June 2017 9,578 1 View

In the ChIP-qPCR assays what is the best method for DNA quantification and how should I normalize my data?

I started using ChIP in my analysis a short time ago and I have a lot of questions about how to normalize my data, what percentage of Input should I use and what is the best method to quantify my...

14 June 2017 7,259 3 View

Is there a model for ZF in which a topological group can fail to be completely regular?

01 June 2016 1,442 2 View

Is there any existing method, protocol or kit for gamma-glutamylcysteine synthetase assay in animal tissue?

I know about ELISA kit but we are more interested in kinetic or absorbance measurement.

08 July 2015 9,509 1 View

How strongly does Hilbert projection theorem depend on CC?

01 January 1970 6,675 3 View

Research "National Identity in Food Culture on Social Media Platforms"

01 January 1970 8,420 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View