Does anyone have a simple method for preparation and analysis of per-methlyated alditol acetates?

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Do you have maps of Ba Vi National Park in Vietnam (Conservation area and buffer zone maps)?

I am currently conducting research on the Ba Vi National Park in Vietnam and need a map delineating the conservation area and its associated buffer zone. - this is surprisingly very hard to find -...

06 April 2024 1,320 4 View

Does anyone know who I can email for permission to use the Survey of Perceived Organizational Support (SPOS)?

I am not sure if permission is needed but I am having trouble finding the holder of the copyright for the Survey of Perceived Organizational Support (SPOS) developed by Eisenberger et al. (1986),...

14 January 2024 6,153 1 View

Has anyone compared Miltenyi's and Stem Cell Technologies' MACS reagents and magnets for CNS tissue?

I've had good luck with Miltenyi's column-based MACS isolation of glial cells, but I'm wondering how Stem Cell Technologies' EasySep method compares since it does not involve columns or the force...

15 October 2023 6,768 0 View

How do you utilize detergent in sonication of tissue homogenates designed to release virus or VLPs?

Determining impact of NP-40 and Freeze/Thaw cycles

08 June 2022 925 0 View

What detergent should be used to release viral particles from tissue without damaging the virus (for titration purposes); as in along with sonication?

Virus titration. Detergents.

25 May 2022 9,789 1 View

Protein quantification of cell lysate in urea buffer?

We need to do our protein extractions in urea lysis buffer (recipe below) because SWI/SNF complex members bind very tightly to the DNA and won't show up on a western with just RIPA lysates. Is...

05 April 2022 2,220 2 View

Alternative to Leibovitz's L-15 Medium for CAOV4 Cells?

We recently purchased CAOV4 ovarian cancer cell line from ATCC which requires growth in Leibovitz's L-15 medium. This medium is designed to be used without CO2. Does anyone have experience using...

11 February 2022 2,856 0 View

Minimum sample size (real observations) for bootstrap?

I am looking for papers / sources that outline any kind of general rule for a minimum sample size (real observations) for using the bootstrap. For now I am using it to generate the CI for a...

09 August 2021 2,091 3 View

Problems with Running Stage of Western Blot?

Wondering if anyone has any insight on why my gels are not running well? They are bowing in the center when running and it constantly switches itself off (the power cell will say that the load is...

15 July 2021 8,246 0 View

Is there a way to automatically gather surface area from a 3D model based on color/texture?

I have been constructing 3D models of large corals and I was wondering if there is a software that I can use to gather the area of live tissue vs dead/disease? I am aware there is a way by tracing...

17 December 2020 7,517 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Robin Joshi

You can seen our paper of tea seed saponins.

In vitro cytotoxicity, antimicrobial, and metal-chelating activity of triterpene

saponins from tea seed grown in Kangra valley, India

Terri Grassby

I have a full description in my thesis "Phenolics and phenolic-polysaccharide linkages in Chinese water chestnut", which can be accessed here: https://ueaeprints.uea.ac.uk/33057/#!

Kristin Whitney

Thank you both for your responses. I will look into both of those methods.