Can anyone recommend a RNA extraction protocol/paper references for mature sweet cherry fruits?
The rapid CTAB method can give high-quality RNA in only 3 h at low cost with efficiency equal to or higher than that obtained with other time-consuming and expensive protocols. The procedure is applied to RNA extraction from grapevine, cherry, olive, lemon, poplar, chestnut, apple, pear, peach, apricot, plum and kiwi fruit. RNA of high quality can be isolated from all tissues and from all species.
Please follow the paper below for details
Hi. On general I am using Trizol reagent for total RNA extraction and on general it is working quite well, with very good RNA quality and yield, and almost no DNA contamination. Often before when i use CTAB method (it is good one) I have sometimes a lot DNA contamination and I do not like to treat my RNA with DNAseI.
Good luck!
yes, you can try TRizol also. I extracted RNA from root and leaf of rice plant using TRIzol (Invitrogen, Carlsbad, USA) following the manufacturer’s instructions and this RNA was subsequently used for first strand cDNA synthesis with the aid of Superscript ΙΙ (Invitrogen) using oligo dT primers.
The TRIzol (Invitrogen, Carlsbad, USA) was found good for isolation of sufficient amount of RNA.
Why negative vote? Down-vote does not explain your (down voter) answer. Please write your answer so that we can learn from you also. Please let us know your views.
But I feel happy as your down-vote helps me find out my lacking.
Thanks
If you can afford it buy a kit for RNA extraction, a kit for DNAase treatment and a kit for cDNA synthesis and follow the instructions. The latter two kits are more or less necessary and you can only perform RNA extraction without a kit. There are also some tricks that you can use during the process that me (and I believe anybody in the forum) can discuss when you need to.
- Badges
- Science method