Does anyone have a reliable protocol for extracting mitochondrial DNA from cells?

06 September 2018 1 8K Report

I am looking to extract mtDNA to run on a simple agarose gel. Protocols online are contradictory and seem to jump between talking about cells to talking about tissue and back.

Craig S Wilding

For pure mtDNA you will need to undertake CsCl centrifugation. You can get a good extraction of mitochondria which can then be lysed using a sucrose centrifugation. Tjis worked well for me many years ago https://www.researchgate.net/publication/13941623_Mitochondrial_DNA_variation_in_the_scallop_Pecten_maximus_L_assessed_by_a_PCR-RFLP_method

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