Hi,
you can use an imprinting method with collodion, which was already made on rice: http://link.springer.com/article/10.1007/PL00006972
Hope this helps
You can visit our recent publication on stomatal closure study in rice mutant under water deficit stress. Below is the link.
http://aobpla.oxfordjournals.org/content/early/2015/03/27/aobpla.plv023.full.pdf+html
Biocompatible polyvinylsiloxane dental resin impression can be a choice, but care to select the same active leaf portion; in monocots leaf blade age grows with distance from the stalk. https://www.colby.edu/academics_cs/courses/BI214/upload/lab5-stomata.pdf . Observation with a steady state porometer would be much less laborious and can apportion the dynamics of the same leaf area under treatment.as in http://jxb.oxfordjournals.org/content/57/2/373.full
you can easily measure stomata opening and closing by (Infra Red Gas Analyzer) IRGA !!
Please have a look on the following publications which used indirect method for finding stomatal opening and closure (PSII efficiency under non-photorespiratory conditions). If you used this method it give you also a whole picture for stomatal status of whole leaf
Aliniaeifard, S., Malcolm Matamoros, P., van Meeteren, U., 2014. Stomatal malfunctioning under low Vapor Pressure Deficit (VPD) conditions: Induced by alterations in stomatal morphology and leaf anatomy or in the ABA signaling? Physiol. Plant 152, 688-699.
Aliniaeifard, S., van Meeteren, U., 2014. Natural variation in stomatal response to closing stimuli among Arabidopsis thaliana accessions after exposure to low VPD as a tool to recognize the mechanism of disturbed stomatal functioning. J. Exp. Bot. 65, 6529-6542.
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