18 Questions 21 Answers 0 Followers
Questions related from Tuyet Van
Preparation sample for SAM, samples were fixed with glutaraldehyde and then post-fixed with OsO4. OsO4 is extremely toxic to human and damage proteins and other components of sample. Could I skip...
15 July 2016 3,369 4 View
I am starting working on CRISPR-Cas9 on rice. Firstly, I want to design primer, but I still wonder about how. Let me list the thing I know about design primer in this case: - 20bp size. - Follow...
11 November 2015 2,933 4 View
I am working on rice and I want to do ROS test to conform my gene function. Please share with me any protocol or paper which relate to ROS test. Thank you in advance.
27 October 2015 652 4 View
Does anyone have any information on the different sizes of Indica and Japonica flag leaves? Thank you in advance.
22 April 2015 5,232 2 View
Thank you in advance.
09 April 2015 4,565 6 View
I am working on the gene which has DNA sequence. However, database do not support full length cDNA (just a part of it can be identified and database announce that ATG can not be detected). How can...
18 March 2015 8,210 4 View
I am working on rice. I found a difference in leaf between F1 wild type and F1 homozygous plant. However, when I germinate F1 heterozygous, some homozygous plant shows the phenotype in leaf and...
16 December 2014 5,009 5 View
I am doing cloning with pBD-GAL4 Cam. This vector has 4 unique restriction sites in the MCS (EcoRI, SalI, PstI, SrfI). My fragment also has SalI, PstI restriction sites, then I must do cloning...
31 October 2014 7,504 3 View
I am working on the T-DNA mutant line. I germinated seeds for about 7 days and genotyped all seedling except for the ungerminated seeds. All seedlings are wild type and heterozygote plants. T-DNA...
22 October 2014 3,727 6 View
I am having trouble with designing a primer for cloning Y2H. My full length RNA sequence from source lacks 5'-UTR region, it starts at ATG, then I must design a primer from ATG. The trouble is...
15 October 2014 4,507 4 View
I want to conform T-DNA insertion cause abnormal embryo, then extracting DNA is needed. Please help me!
01 October 2014 7,839 5 View
How can sampling rice pollen at unicellular, bicellular and tricellular for RNA extraction? Anyone has experience about this work, please help.
18 August 2014 5,951 2 View
For hormone treatment in short term (around 6-12 hours), 100uM jasmonic acid/methyl jasmonate is enough to induce plant. In case in a long term (7-10 days), which concentration is suitable for rice?
30 May 2014 356 1 View
I have found dwarf phenotype, cosegregate to T-DNA insertion. My problem is: 1. T_DNA insertion (pGA2715 vector) locates between 2 genes(A and B), upstream of A around 82bp, expression level of A...
21 April 2014 9,178 3 View
It must be high concentration of primer for iPCR or tail-PCR, but that's too high, almost 1.000.000 times higher than usual. How concentration can be used for iPCR or tail-PCR, it can be around...
27 February 2014 3,773 5 View
I would like to make the promoter comparison of one gene between Japonica and Indica cultivar.
27 January 2014 1,316 9 View
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10 December 2013 1,604 2 View
I'm looking for the gene which can be a positive control under drought conditions for real time PCR in rice. I found some papers about drought stress in rice but most of them do not use a positive...
13 May 2013 6,221 3 View
