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Questions related from Tuyet Van
Does anyone have any information on the different sizes of Indica and Japonica flag leaves? Thank you in advance.
22 April 2015 5,139 2 View
I am working on the gene which has DNA sequence. However, database do not support full length cDNA (just a part of it can be identified and database announce that ATG can not be detected). How can...
18 March 2015 8,157 4 View
I am working on the T-DNA mutant line. I germinated seeds for about 7 days and genotyped all seedling except for the ungerminated seeds. All seedlings are wild type and heterozygote plants. T-DNA...
22 October 2014 3,682 6 View
I am having trouble with designing a primer for cloning Y2H. My full length RNA sequence from source lacks 5'-UTR region, it starts at ATG, then I must design a primer from ATG. The trouble is...
15 October 2014 4,461 4 View
For hormone treatment in short term (around 6-12 hours), 100uM jasmonic acid/methyl jasmonate is enough to induce plant. In case in a long term (7-10 days), which concentration is suitable for rice?
30 May 2014 229 1 View
I have found dwarf phenotype, cosegregate to T-DNA insertion. My problem is: 1. T_DNA insertion (pGA2715 vector) locates between 2 genes(A and B), upstream of A around 82bp, expression level of A...
21 April 2014 9,129 3 View
It must be high concentration of primer for iPCR or tail-PCR, but that's too high, almost 1.000.000 times higher than usual. How concentration can be used for iPCR or tail-PCR, it can be around...
27 February 2014 3,728 5 View
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10 December 2013 1,564 2 View
I'm looking for the gene which can be a positive control under drought conditions for real time PCR in rice. I found some papers about drought stress in rice but most of them do not use a positive...
13 May 2013 6,170 3 View