We are working with nanoparticles into which we wish to load with microRNA precursors. Since buying commercially synthesized precursors of microRNA is quite expensive I was wondering if it would be possible to synthesize it myself.
Leonardo Chicaybam
It is only a guess, but in vitro transcription (IVT) should work...there are several protocols that work very well (assuming that you have the shRNA cloned in a plasmid).
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