I am interested to do the purification of this hormone
Dear Marsilda Qyli
Go for = http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0134983#pone.0134983.ref039
and https://www.ncbi.nlm.nih.gov/pubmed/2449095
Pls. find the attached files
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2756545/
http://link.springer.com/article/10.1007/BF00692738
http://rspb.royalsocietypublishing.org/content/251/1330/53.short
https://icb.oxfordjournals.org/content/46/6/965.full
http://www.journals.uchicago.edu/doi/pdfplus/10.2307/1542327
http://www.sciencedirect.com/science/article/pii/0014579389810518
http://www.nature.com/articles/srep17055
http://link.springer.com/article/10.1007/s12562-010-0247-1
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3256185/
http://molbiol.ru/forums/index.php?act=Attach&type=post&id=92824
http://europepmc.org/abstract/MED/2449095
http://www.ijsr.net/archive/v3i8/MDIwMTU0MDk%3D.pdf
You may also contact the researcher of the article listed
Hoping this will be helpful
Regards
Prof. Houda Kawas
Dear Prof.Houda!
Thank you a lot for your information,
Best regards,
Marsilda
You most welcome
Houda KAWAS
I should use it for the purification of the cHH hormone in crabs
09 October 2016 9,967 2 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...
05 August 2024 9,805 3 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View
I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...
16 July 2024 4,604 0 View