I plan to measure residual conductivity of the cell culture as a parameter of growth. However, not quite sure how to do it exactly. I would be appreciated if you share such protocol, text ,etc in this case.
Thank you.
Our lab measures cell monolayer resistance with a four point probe. This can be used to indicate confluence of the cells and even indicate the presence of tight junction formation in endothelial cells. These measurements generally need to be done with an AC current applied to two probes that are on opposite sides of the membrane while measuring the voltage from the other two electrodes (also on opposite sides of the membrane). There are some specialized instruments that can do this (EVOM2 from WPI) or you can build your own if your into that. Our lab is currently working on building our own custom electronics for measuring Trans-Endothelial Electrical Resistance (TEER) in our microfluidic devices. You can search for TEER measurement and find a great number of papers that will give you useful information about measuring resistance in cell culture. Also look at the flocel.com website. Dr. Damir Janigro has about a dozed papers that utilize resistance measurements in the publications section.
Most of the companies that make these devices can provide starting points for protocols but you will need to optimize your process based on your cell type and the specific physiological requirements of your experiments.
If you can provide more details about your cell type and experimental requirements, myself and others will be able to provide more detailed information.
Dear Ron,
Thank you ver much for your perfect explanation. The cells I'm working on are BY-2and NT1 plant cell lines. They are very fast growing cells. I will have different concentrations of a chemical material and have them under different abiotic stresses while they are growing. then I need to measure different parameters like electrical conductivity of each treatment. Preferably, I let them grow in 48 tissue culture well plate. I don't know whether that volume is enough for this measurement or I need more volume?
Regards
Our company develops 3D cell barrier culture systems for in vitro study of virtually any cell barrier, and the system comes equipped with both a Fluid Perfusion Unit and a Trans-Endothelial/Epithelial Electrical Resistance (TEER) Measurement Unit! The use of a vessel-shaped 3D environment has been proven more effective than 2D systems like Transwell (
Article Santaguida S, Janigro D, Hossain M, Oby E, Rapp E, Cucullo L...
), and our advanced TEER Measurement Unit allows for frequency sweeps between 0.1-1,000 Hz, automated time point sampling, logging of data to Excel, and additional measurement of phase angle for cell capacitance calculations! For those that are committed to Transwell use, we also have a TEER Measurement Unit that is compatible with nearly all Transwell products (Endohm cell cup chamber, STX2 "chopstick" electrodes, etc) and allows the user to greatly expand their testing capabilities with Transwell equipment. Additionally, we have smaller modular systems that connect to microscope-friendly cell culture modules: i.e., a miniaturized 3D cell culture system that you can use right under your microscope! If you or anybody on this thread is interested in learning more, I encourage you to visit www.flocel.com or email me at [email protected], thank you!
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