Does anyone have a protocol for c-Fos labeling in cleared (CLARITY) whole mouse brain?

18 August 2020 1 4K Report

This is a first run in our lab and there are several variables that each add several days to the protocol. I will be using the DeepLabel Antibody Staining Kit from LogosBio to label c-Fos. Questions are:

Which primary antibody do you use? At what concentration? How long do you incubate?

What concentration of secondary antibody do you use? How long is this incubation?

If anyone would share experience/protocol that would be great! We have been clearing and imaging brain and other peripheral tissues as well so if we can be of any assistance, please feel free to contact me.

Katharina Jannasch

In their latest publication in STAR protocols (PMID 38280198), Refaeli et al. published a detailed protocol for marking engram cells using a modified version of CLARITY in combination with a rabbit monoclonal c-Fos antibody (Synaptic Systems #226 008 [https://www.sysy.com/product/226008]).

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