What is it? Subject classification codes?

Dear colleagues, help me to understand the rules for authors. I am writing an article for journal "Climacteric". According to the rules for autors it is necessary to indicate "Subject...

09 February 2021 2,221 4 View

How to analyse multiple Likert scales?

Hello everyone! I've encountered a problem when trying to figure out the methodology of my dissertation. The study has multiple variables and will be, in general, rather complicated, but my only...

02 February 2021 2,086 3 View

Variable with negative values in SPSS?

Hello, dear colleagues! I would ask you for a little help. Actually, I'm doing a multivariate linear regression analysis in the SPSS and one of the independent variables has negative values. In...

20 December 2020 535 2 View

ICP-Analysis: how can I consider the loss of sample mass after sample filtration?

To prepare a sample for ICP-Analysis one has to dissolve the material in the chosen liquid and then filter it. How can I consider the loss of mass after filtration if I don't know it exactly? I...

20 December 2020 4,394 3 View

How to report results from piecewise structural equation modelling analysis?

I have done my analysis in R using the piecewiseSEM package and have got the results but I am unsure how to report it APA style. What values do I report? Thank you in advance!

13 December 2020 3,048 3 View

Is USDA NASS plot-level Objective Yield Survey data publicly available?

I'm interested in the results of Objective Yield Survey by USDA NASS in a raw form of plot-level observations. As far, as I understand, quickstats...

19 November 2020 5,369 3 View

What is the most sufficient way of protein immobilization on SiO2?

I need to immobilize 3 peptides: CGPDGRDGRDGRDGR, CRQILIWFQNRRMKWKKYQRLC, penetratin peptide RQIKIWFQNRRMKWKKGG on the SiO2-covered gold nanoparticles. What is the most efficient and stable way...

16 November 2020 2,909 3 View

How to dissolve betulin in DMSO (20mM stock solution)?

I would be very grateful for any help regarding my problem. My research requires applying betulin (and betulinic acid) solution on the cerebral cortex slices of rats. With the stock solution of...

27 September 2020 8,758 2 View

Scanning Electron Microscope- How to correct crossover?

Hello, I have questions about XL30 SEM (with tungsten filament). Can anybody help me with how to correct crossover? You will see in the picture look of my crossover. I trying everything and can't...

24 May 2020 1,339 3 View

Is it possible to precisely evaluate a thickness of a NiP-Cr coating on a stailness steel with XRF?

Hello everybody, I have a qestion about the implementation of the XRF to determine the thickness of the NiP -Cr coating on a stainless steel (1.4305). According to the TDS of the base Material,...

24 February 2020 5,574 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View