I would like to know if anyone has a pHT304 vector. I need to use it asap for gene complementation in Bacillus cereus.
I will be grateful to get a reply soon.
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I am trying to coating metal oxide into steel substrate, for better sticking planning to utilse epoxy based, Could you please suggest suitable curing agent for the coating purpose
25 July 2024 438 3 View
I am experiencing this for the Bi2Se3 sputtering target. Initially, even if the target was 2:3 in Bi : Se, with a few sputtering runs, due to the high escaping tendency of selenium, the top layer...
23 July 2024 1,649 1 View
I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...
20 July 2024 1,339 2 View
I want to know the best methods to automate my job and live without working?
12 July 2024 5,993 2 View
I would like know what are the best ways to earn more money as a researcher?
11 July 2024 4,695 2 View
I would like to know how do I monitor my CCTV camera at my home in Bangalore from my current location which is California in USA?
11 July 2024 831 0 View
I would like to know how to check if a given research work is human made or AI generated?
11 July 2024 8,645 5 View
I am interested in knowing how does an AI detection tool work and what's the main logic behind it? How it is able to detect the presence of AI work?
11 July 2024 3,889 6 View
I would like to know how to prove my research work is new and not copied from other people's work or AI tool?
09 July 2024 8,235 2 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View