I’m working on spinal cord mice tissue and I want to satin motor neurons with luxol blue and cresyl violet, so does anyone have a working protocol?
I have a working protocol for Crasyl Violet Stain Solution (CV), i use it for mice's brain tissue after mounting the slices in the slides (the slices must be dry)
CV Percentage: 0,1%
1) Incubate slide in CV Solution for 4-5 miutes
2) Rinse in distilled water for 1 minute
3) Dehydrate in 50% alcohol (1 min) 70% alcohol (1 mini) 96% alcohol (3 min) and two passages in absolute alcohol (3 min each)
4) Clear in Xylene (1 min)
5) Coverslip quickly
of course, it really depends on the kit you use
I have tried the solarbio kit and the atomscientific one
both were providing good results
generally, first, you have to dewax your sections, hydrate with 95% alcohol
the stain in LFB for 2 hrs at 60 degrees Celcius or overnight at room temp.
then rinse with DW, then differentiate with a specific differentiator that comes with the kit and continue to differentiate with 70% alcohol, check the slide under microscope to check the differentiation
finally incubate slide in CV stain
wash with DW, dehydrate with alcohol, and mount.
hope this was helpful
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