I want to Immunostain retina vasculature, so I'm looking for a protocol to embed the retina in wax, do I need to embed and section the whole eye or do I have to remove the cornea and the lens and embed the eye cup and is there any tips on how to handle the retina during the embedding????
From experience, hard tissues such as the lens should be dissected before embedding if they are not your target tissues and if you do not need to keep the eye shape. If you are worried about handling the retina during embedding you can leave the sclera during this procedure and trim it from the block later or just keep it if you can still get good sections from it.
Yes, it would be good if you could isolate the lens, since it could break while sectioning, and breaking the rest of the sections.
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