What is the chromatographic technique used for the Gentamicin quantification?

HI everyone, I want to quantify the gentamicin antibiotic presenting in the culture medium. Can You anybody please suggest me suitable chromatographic technique for that? Please suggest me the...

16 December 2020 9,823 3 View

Principal Component Analysis?

Hi everyone, I want to perform Principal component analysis (PCA) for Excitation Emission Matrix (EEM). Can You anyone give the python language coding for it?...

17 November 2020 2,776 5 View

How do we interpret the following gender differentiated data?

Hi, We are analyzing data and have found the following finding. The results show that men and women hold equal responsibilities for many aspects of forest management and decision making in the...

27 October 2020 6,399 4 View

Can someone help me with different watershed management practices for hilly areas ? Requirement is to have enough water for crop cultivation?

Can someone help me with different watershed management practices for hilly areas ? Requirement is to have enough water for crop cultivation. I am looking at the hilly areas, any proof of concept...

05 October 2020 5,641 15 View

Please, i want to learn about UV graph ?

the blue circle in uv graph can consider as the head of graph and can i take this points the value of the wavelength of nanoparticles ? in the photo with number 1 this graph indicates that there...

09 August 2019 158 1 View

Hello, dear researchers. I'd like ask about your opinion about this UV graph?

first image for SNPs synthesized by certain plant, while second image is the UV of plant extract without silver nitrate i think the wavelength of SNPs start from 300 to 450 nm? thank you

06 August 2019 5,311 2 View

Can anyone suggest from where I can get Microgrid data, such as nodes load, impedance etc ?

I'm working on Microgrid load management.

17 October 2016 8,272 5 View

Project Inputs

Hi, I am doing Ph.D in IT and specialized in Information Secuirty. My current project is Artificial Intelligence in Export System. I am expecting some inputs from your. So kindly help me. RS...

05 November 2010 5,483 0 View

What is meant by atmospheric river?

my project is based on atmospheric water so,i think it will be helpful for me.if i know about this

01 January 1970 6,112 5 View

Ethnobotany Theory - titles that further critical thinking and the development of the discipline of ethnobotany

01 January 1970 900 7 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

No title

23 February 2021 4,014 3 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Peptide precipitation as pre-step for acid hydrolysis for amino acid analysis?

I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...

14 February 2021 6,049 3 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

What is the optimal propagation regime for pET-1M(KanR) and pMCSG7(AmpR) plasmids in DH5alpha E.coli?

Dear all, I am trying to harvest the low-expression plasmids in the mentioned E.coli strain. I constantly gel very low concentrations (30-50 ng/uL while the concentration is usually around 100...

08 February 2021 4,255 4 View