Are there any corporations that have launched Enterprise MOOCs to the public?

I am studying Enterprise MOOCs and can't seem to locate very many empirical articles for this area. The model I am interested in is similar to openSAP (https://open.sap.com). Please share any...

28 September 2020 6,813 2 View

Any insights on using USGS water quality data (ie total dissolved solids) in a statistical analysis when samples are dried at different temperatures?

11 December 2016 3,264 8 View

How do you include a theoretical framework in a research proposal for an exploratory study on knowledge and beliefs?

I am a Honours student, applying for the masters programme in counselling psychology. I am expected to produce a research proposal for my possible master's thesis. I have decided on doing an...

20 August 2016 9,989 11 View

Does anyone have knowledge pertaining to expressing bacterial proteins in mammalian cell culture? Do you need to worry about codon usage?

We have created a modified toxin plasmid using a mammalian receptor domain. We are trying to express the modified toxin plasmid in cell culture, and isolate the protein. We need mammalian cell...

08 June 2015 186 3 View

What brands of Ni-NTA resin work best?

I am doing a gravity purification of 6x-His tagged staph nuclease in a pET28b+vector under native conditions, with no special conditions. This protein is very easy to purify and usually boatloads...

19 May 2014 2,517 10 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View

Rat Macrophage Markers for Flow Cytometry?

Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.

04 February 2021 3,913 4 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

How to do rat bone Immunofluorescence, Please suggest?

Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo

26 January 2021 4,165 3 View

The litter effect and what can I do to avoid it?

My thesis involves experimental study with Wistar puppies. I am dividing the puppies equally in relation to the groups. However I only have 4 mothers and I will use all of their puppies. There is...

25 January 2021 6,965 3 View