Transformation of Bacillus subtilis?

Colonies appeared after transformation of pHT01 plasmid into Bacillus subtilis, and band was confirmed by performing colony PCR. However, if the colony is picked and grown (in LB+antibiotics) and...

27 October 2020 609 4 View

Is this correct molecular docking?

Hello, buds I need your help to interpret the attached data. R group in serine is CH2OH, but the data show the two O in the group with hydrogen bonds with a molecule. Is this correct?...

02 October 2020 7,787 6 View

Dataset size and the need for cross-validation (medical domain)?

I am currently envisioning a deep-learning model that classifies pulse waveforms (normal vs. pathologic). At this time, the no. of pulse dataset is about 2,000,000, and the balance of normal and...

31 July 2020 6,713 3 View

[ELI5] FRET assay, Protease assay, Michelis Menten. How would you convert the fluorescence signal to the velocity (concentrion/time)?

Hello i am fairly new to the field of physical chemistry and only have a vague memory of it from undergrad classes. my FRET assay is set up like a regular FRET assay, so the enzymetic activity of...

18 June 2020 9,730 4 View

Do buffers need to match for DNA (aptamer) to protein binding in SPR on NTA chip?

Hello, As a newbie to SPR, I've been having problems with optimizing aptamer-protein binding on an NTA chip. My protein has a his-tag on it. Any help would be greatly appreciated. Question 1:...

14 June 2020 4,586 3 View

How can I interpret this SEM image?

This SEM images are Li doped Cu2O thin films. Precursor of Li is LiCl(Lithium Chloride). I think there is no crystallinity. Is this correct?

31 July 2019 7,787 4 View

How can we calculate the dead cells in a specific population of Candida cells ?

How can we calculate the exact number of the dead cells in a specific population of Candida albicans cells, with or without staining ? If a dye were used, which one is better ?

27 November 2018 4,432 3 View

Does anyone know the BRCA1 status of the prostate cancer cell line 22Rv1?

ATCC catalog number is CRL-2505

06 November 2018 5,697 3 View

Problem of urine LTE4 level in ELISA test?

14 October 2018 4,494 1 View

Does anyone have a good reference for gestational sizes of Sprague Dawley rats?

Looking for data on gestational sizes of SD rats and also if an atlas of development exists. Thank you!

12 April 2018 6,819 1 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Why is My firend's profile not visible?

Hello, I created a profile for my friend on google scholar. I have added a verified email address. I marked the profile as public. Formerly his profile was visible. However, now, When I type his...

24 February 2021 8,680 3 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

22 February 2021 3,894 6 View

I can't edit my publication, the "edit" is blurred. How can I edit it?

I have entered a wrong question mark in my publication and added "Forthcoming", I want to edit the title of my publication, but the "EDIT" is blurred, I can't edit it. Any suggestion? thank you.

18 February 2021 1,279 3 View

How to decrease the time for publication of paper in a journal ?

It takes a lot of time in publishing the paper in the journal, from few months to a year so how one can able to decrease the time for publication.

17 February 2021 2,997 14 View

Does anybody know a public dataset of drop landing or jump landing maneuvers?

Dear all, In our project we would like to test different control strategies during drop landing compared to jump landing. After an intensive search I could not find a public dataset of drop...

09 February 2021 7,093 4 View