Hi All,

Does anyone have a good protocol for staining bacterial cells (in suspension culture) with a primary Ab followed by a fluorescently-conjugated secondary Ab? I'm just wondering primarily how to get the cells on the slide, fix them (if required), and what types of washes are good. The protein I'm trying to label is extracellular so I don't think a permeabilzation step is needed.

Thanks a lot,

Adam

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