Hi All,

Does anyone have a preferred orientation in which to section mouse small intestine so that one maximizes the number of intact villi in the section? I have tried paraffin-embedding the entire "tube" and cutting transverse sections but with little success. I have now fixed sections where I have cut the tube open such that the villi should all be on one face of the section and the mucosae muscularis on the opposite face. What is the best way to orient this type of section in paraffin to see the most villi? Thank you in advance for your help.

Best,

Adam