Does anyone have a good protocol for isolating (separately) DNA and RNA from brain tissue?

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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Are protein samples in SDS compatible with ELISA?

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Fulvio Celsi

the best and quick answer is: separate your tissue! :) I mean that for example, you have two hippocampus, one for DNA and the other for RNA...

If this is not feasible, then go for Trizol (Tri-Reagent -SIgma). by using it, you could separate first RNA, then DNA (also protein but then it is tricky). Trizol is quite useful anf in my experience you got good yeld of RNA and DNA...here is the link from Sigma

http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/t3809bul.Par.0001.File.tmp/t3809bul.pdf

Gary David Isaacs

Thanks for the input Fulvio. I was thinking about using Trizol over the Qiagen kits but my experience has only been with the RNA isolation. I may take your suggestion about separating the tissue but I'll check the yields on the dual method first. thanks again!

Jean-Nicolas Audet

It seems that Ambion (now sold via Life) got what you need: http://www.invitrogen.com/site/us/en/home/References/Ambion-Tech-Support/rna-isolation/tech-notes/simultaneous-extraction-of-rna-and-dna.html

Thanks Jean-Nicolas. That looks like a modified Trizol protocol so I copied down their extraction buffer recipe for comparison.