I have used to to three protocols but very minute quantity with high impurity DNA extracted, so i need little more good protocol.
Liquid nitrogen is not needed at all for DNA isolation, it is just very useful to quickly freeze samples until they can be used for extraction. It is also possible to dry leaf samples by collecting them in a small sealable plastic bag filled with a tea spoon (depending on the size of the leaf of course) of dry silica gel. Samples stay fine for several months at least, provided the bag is properly sealed.
http://www.jstor.org/stable/1222975?seq=1#page_scan_tab_contents
Fresh material can be used straight away for DNA extraction.
Storing samples in presence of silica gel is a very simple way and usually works for many types of samples.
Simply use fresh biological material for DNA extraction. Use grinder to crush your leaves and then use CTAB extraction protocol. I use it for most of my DNA extraction from various plant species. Its usually very clean.
You can lyophilize the samples, and stored then for at least a year (in a dry place, with silica gel), and then use a CTAB method for extraction.
Yes. Please go through our article-Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA
markers to study genetic diversity of Indian Garcinia. at African Journal of Biotechnology -Vol. 12(46), pp. 6443-6451, 13 November, 2013.
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