Jigarkumar Suthar

11 Questions 20 Answers 0 Followers

Questions related from Jigarkumar Suthar

Is the universal + repeat sequence primer (p4) with fluorescent label can work for TP PCR in case of friedreich ataxia ...???

I have gone through multiple research article for TP PCR based diagnosis of Friedreich ataxia. the majority of paper who shows the basic concept of tp pcr are telling to label target specific...

15 September 2022 239 0 View

Need help regarding Intron 22 inversion long range PCR protocol.?

I have ordered platinum Super Fi II mastermix from thermo scientific for this long range PCR. i am using the primers from Bagnall et al 2005. now i am getting the 14 to 15 kb band in place of 10kb...

20 March 2021 778 3 View

Can anybody suggest me that the correlation between volume of sample loaded on the Chip (NGS chip) Vs reads generates ...?

I need to know that how can ve decide that this sampke needs 1 million reads so we have to load this much sample, where the otherone sampke needs 200k reads then it would be loaded the xyz.

21 April 2019 6,976 4 View

Anybody can suggest a good Giemsa trypsin banding protocol ?

I am using SSC buffer to make a giemsa (20mg per 50 ml SSC buffer). we use aged slide (65 degree for overnight) for treating with Trypsin Solution for 20 sec at 20 degree. We get minor banding...

02 August 2018 2,653 3 View

RAPD analysis of binomial matrix and dendogram preparation?

I have two variety, one is A - in vivo plant leaf and B - in vitro plant leaf. i had extracted DNA from these both samples A & B. Done PCR with 31 RAPD primers and got varies band pattern on...

09 September 2016 7,910 5 View

RAPD analysis for the in-vitro and in-vivo plant samples of the same plant species?

I have done RAPD Analysis for one plant with its Invivo tissue and in vitro tissue samples. I have used 31 primer sets, so now i dont getting how much maximum variation can be considered for this...

20 August 2016 871 4 View

Which criteria should be considered for converting the Band pattern on gel of RAPD in to binary pattern for making dendogram?

i have got Band pattern on agarose gel, now i need to analysis this results and have to prepare dendogram from this results, so what should i do? What do polymorphic and monomorphic bands means in...

07 August 2016 3,952 4 View

Which HPLC method, column type and solvent system will best suite for Alginate analysis extracted from different bacterial strains?

i have extracted Alginate by growing different bacterial strains in broth media. after extracting now have dried alginate as per the extraction protocol. Now i want to check its purity and want...

28 June 2016 7,628 3 View

How can I remove a high polysaccharide content from DNA extracted with plant leaf?

I have extracted plant DNA from leaf sample. i got good precipitates when precipitated with ethanol and iso propanol. but when i dissolve the dried pellet in TE or molecular grade water, it became...

20 December 2015 9,506 3 View

Does anyone have a good DNA extraction protocol from plant leaves without using liquid nitrogen?

I have used to to three protocols but very minute quantity with high impurity DNA extracted, so i need little more good protocol.

31 August 2015 8,237 7 View

Can anyone suggest end point PCR kit for HPV DNA screening, TB PCR, Cystic fibrosis mutations and beta Thalassemia & sickle cell anemia?

i want to start molecular diagnosis unit in my lab. so i need highly specific kits for different genetic disease. I don't have Real-Time PCR, I just have conventional gradient PCR machine, so i...

17 August 2015 8,043 2 View