Does anyone have a good company for ordering lentiviral GFP vectors?

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How to properly dilute RNA for cDNA?

Hi all, I have a question regarding proper dilutions and conversions of my RNA. According to my protocol, my cDNA kit is optimized for 1ug of RNA and calls for this amount. I would like to...

07 July 2024 9,206 4 View

Can I use BSA for a Histological Blocking Solution?

Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...

07 July 2024 7,967 3 View

Research on black and ethnic minorities' detained under the mental health act ?

research on mental health and ethnicity

18 June 2024 3,958 0 View

How to analyse category membership changes over time?

I have a longitudinal dataset where I am looking to examine whether individuals change their category membership of a variable (with four categories) over four time points (baseline, 1y, 2y, 3y)....

16 June 2024 9,770 10 View

Luciferase assay lysis?

Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...

13 June 2024 9,936 5 View

What are good EBP's or therapies for the executive function deficits that kids exhibit after PTSD diagnosis?

I've worked with several children in classroom settings who have trauma backgrounds, and they're academically average or above average. They present ADHD, but because they don't have IEP's,...

20 April 2024 4,059 1 View

Tips for using SpeedVac to concentrate DNA?

Hello! I eluted by DNA in 100ul of AE. Some of my concentrations are low, and I am out of tissue so I'd like to concentrate using the SpeedVac, but I'm worried about concentrating the salts in the...

14 January 2024 2,657 0 View

Please click on link below if you are in the healthcare setting in the United States?

I'd love to hear from you about your experience working under leadership in the healthcare setting! We are looking for professionals across the United States who have worked or are currently...

05 December 2023 7,868 0 View

Will a pap pen adhere to a gelatin coated slide for IHC staining?

The tissue is already mounted on a gelatin coated slide. Is it possible to use a pap pen to provide the barrier for IHC staining?

01 November 2023 6,086 0 View

Is there a way to draw and save multiple free-hand ROIS as TIFFs while also saving the .roi files?

Hi All, I’m new to ImageJ image processing and have a question about drawing multiple custom ROIs on an image and saving each ROI as a distinct TIFF. I’m working with images of a rodent brain,...

31 October 2023 1,355 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View