Does anyone have a good company for ordering lentiviral GFP vectors?

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Data Analysis K-S and S-W tests?

Hello, I have conducted analysis where the K-W and S-W have come out as p

25 February 2021 9,648 3 View

Simple clearing method for observation of hand-sectioned wheat leaves?

I am using a toluidine blue O preparation for staining different parts of wheat plants to observe a fungus growing in them. This works really well for lighter tissues of the plant! However, the...

27 October 2020 9,159 1 View

How to Perform Feature Selection with Multilevel Encoded Categorical Variables?

I'm attempting to do feature selection on the variables on my dataset, but unfortunately the overwhelming majority of them are categorical. Currently they've all been encoded into binary...

29 July 2020 7,581 3 View

3D Slicer to ANSYS or FEBio?

Hi all, Does anyone have a workflow for preparing volumetric meshes in 3D Slicer and importing them into finite element software such as ANSYS or FEBio? Been trying so many approaches but they...

26 July 2020 7,012 3 View

How do I report Multiple Regression Analysis with imputed values SPSS?

I'm conducting multiple regression in SPSS with data where I've replaced missing values using Multiple Imputation. The output gives the pooled values in the coefficients table, but for the model...

10 June 2020 2,069 2 View

Is there a way to set random initial values for occupancy models in R using the RPresence package? Or any documentation file for RPresence?

I am trying to set random initial values for some occupancy models in R, in response to getting some convergence warnings. I am using the RPresence package but cannot find any documentation files...

09 February 2020 3,057 2 View

Removing vitreous from baby rat eyes?

I have been trying to remove the vitreous humour from baby rat eyes (post natal day 15) and have been still having a lot of interference in my IHC staining. Does anyone have a good protocol to...

20 January 2020 4,634 1 View

Colony Formation assay vs Growth assay - do they report different things?

I'm looking at the response of an established cell line to addition of a drug in terms of growth inhibition. Whilst I see a slight growth inhibition when seeding cells 10-25% confluent (I have...

08 December 2019 319 1 View

GDNA stability at 55 degrees?

I was extracting genomic DNA from Streptomyces the previous day and left my gDNA in the oven (55 degrees Celsius) to dry at the very last step. I had to rush off for a seminar and completely...

11 September 2019 9,441 2 View

How does freezing antibiotics result in loss of activity?

I remember my mentor saying before that she decided to refrigerate her gentamycin stock solution instead of freezing it since that was the recommended storage condition. Now, I'm dealing with...

04 August 2019 5,766 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View