Rachel Lieberman

5 Questions 4 Answers 0 Followers

Questions related from Rachel Lieberman

Do murine isolated NK cells in culture need to be trypsinized for passage or are they floating?

I isolated NK cells from a mouse and they seem to stick to the cell culture plate. Should they be trypsinized for passage?

06 October 2016 1,697 3 View

Does anyone have a good company for ordering lentiviral GFP vectors?

Does anyone have a good source/company for ordering lentiviral GFP vectors to be infected into tumor cells, tail vein injected into mice and then FACS sorted for GFP?

23 November 2015 3,267 2 View

Can someone help me calculate drug dilutions and dosing for i.p. injection?

I have an undiluted drug in powder form. I need to give it to my 20 gram mouse at a dose of 10 mg/Kg. The drug is docetaxel and its MW is 807.87922. The drug product sheet says that it is soluble...

23 July 2015 5,068 3 View

Can someone help me with genotyping PCR?

I am trying to genotype my transgenic mouse by following the protocol of a paper published on the original mouse data and cannot get a clear PCR gel. I want to genotype for the wildtype gene...

20 July 2015 8,533 5 View

Can someone help with calculating drug dosage (updated)?

I have an undiluted drug in powder form. I need to give it to my 20 gram mouse at a dose of 10 mg/Kg. The drug is docetaxel and its MW is 807.87922. The drug is Docetaxel and the product sheet...

17 July 2015 5,180 12 View