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I isolated NK cells from a mouse and they seem to stick to the cell culture plate. Should they be trypsinized for passage?
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I would like to do a cytotoxic T lymphocyte assay where I treat lewis lung carcinoma cells in vitro with splenocytes from a transgenic black6 mouse that I believe to be altering immune...
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Does anyone have a good source/company for ordering lentiviral GFP vectors to be infected into tumor cells, tail vein injected into mice and then FACS sorted for GFP?
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I will be treating mice of different genetic backgrounds with carcinogen, fixing their lungs with formalin, storing in 70%EtOH and performing TUNEL assay to determine differing levels of...
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I have an undiluted drug in powder form. I need to give it to my 20 gram mouse at a dose of 10 mg/Kg. The drug is docetaxel and its MW is 807.87922. The drug product sheet says that it is soluble...
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I am trying to genotype my transgenic mouse by following the protocol of a paper published on the original mouse data and cannot get a clear PCR gel. I want to genotype for the wildtype gene...
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I have an undiluted drug in powder form. I need to give it to my 20 gram mouse at a dose of 10 mg/Kg. The drug is docetaxel and its MW is 807.87922. The drug is Docetaxel and the product sheet...
17 July 2015 5,190 12 View
I have a drug diluted to 5 uM. I need to give it to my mouse at a dose of 10 mg/Kg. The drug is docetaxel and its MW is 807.87922. How much of this 5 uM docetaxel should I use if I want to inject...
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